Histology Services- Routine stainings
Hematoxylin (Nuclei, purple) Eosin (Cytoplasm, pink)
Hematoxylin (Nuclei, purple) Eosin (Cytoplasm, pink)
H&E staining on decalcified bone tissue
Nuclei- black Cytoplasm- pale blue Cartilage- red
Granulocyte and Lymphocyte- Giemsa
Indian Ink- Black DAPI- Blue
Nuclei- purple Muscle, cytoplasm, keratin- red Collagen- blue
Nuclei- purple Muscle, cytoplasm, keratin- red Collagen- blue
Nuclei- purple Muscle, cytoplasm, keratin- red Collagen- blue
Red- collagen green- tissue
* Small Intestine- green * Collagen I+III- red
* Pancreas- green * Fibrotic area- red
Histology Services- Non-fluorescence IHC
B3 Tubulin (brown)
CD34 (brown)
aSMA (HRP)- brown HLA (AP)- pink
TUNEL (apoptosis) Development: HRP. Kidney , x100
TNFa (brown)
Neurofilaments (brown)
TNFa (brown)
KI67 (brown)
GFAP (HRP)- brown AQP4 (AP)- pink
14-3-3 (brown)
Ku80 (Apoptosis) human cancer cells in xenograft
GFAP (HRP)- brown AQP4 (AP)- pink
aSMA (brown)
Pan Cytokeratine (brown)
Glucagon (AP)- pink
Histology Services- Fluorescence IHC
* b-Catenin (red) * PCNA- (green)
* GFAP (green). * AQP-4 (red). * DAPI (blue).
* GCSA (Germ Cells Specific Antigen) (red) * Laminin- (pale blue)
* Mesenchyme cells (red) * Cancer cells- (green) * Immuner cells- (pale blue) *Nuclei- DAPI (blue)
* Mucin2 (red) * aSMA- (green) *Nuclei- DAPI (blue)
* Collagen VI (red) * PCNA- (green) *Nuclei- DAPI (blue)
* aSMA (green). * Vimentin (red). * DAPI (blue).
* aSMA (green). * KI-67 (red). * DAPI (blue).
* KI-67 (red) * Actin (green) * DAPI- Nuclei (blue)
* Murine aSMA (green). * Human HLA (red). * DAPI (blue).
* CTHRC1 (green) * Collagen 1 (red) * DAPI- Nuclei (blue)
* NeuN (Neuronal Marker) (green). * DAPI (blue).
* MAP-2 (neuronal cells-their soma & dendrites Marker)- (green). * DAPI (blue).
* Collagen VI (red) * PCNA- (green)
* Collagen (purple), Cy5
* Collagen VI (red) * PCNA- (green)
OPAL Multiplex Immunohistochemistry
Imaging & Analysis
OPAL- Tongue- *β Catenin- Epithel- (green) *γH2Ax- DNA Damage- (red) *Nuclei (blue)
OPAL- Pancreas- *β Catenin- Epithel- (green) *γH2Ax- DNA Damage- (red)
OPAL- Testis- *β Catenin- Epithel (green) *γH2Ax- DNA Damage- (red)
OPAL- Testis- *γH2Ax- DNA Damage- (red) *Col1a1-ECM- (pale blue)
OPAL- Colon- *AQP4- Epithel (green) *Col1a1-ECM- (red) *Nuclei (blue)
OPAL- Colon- *αSMA-Mesenchyme (green) *PCNA-Proliferation (red) *14-3-3-HIPPO cell signaling (pale blue)
OPAL- Colon- *αSMA-Mesenchyme (green) *PCNA-Proliferation (red) *Nuclei (blue)
OPAL- Colon- *αSMA-Mesenchyme (green) *14-3-3-HIPPO cell signaling (pale blue) *Nuclei (blue)
OPAL- Colon- *αSMA-Mesenchyme (green) *PCNA-Proliferation (red) *14-3-3-HIPPO cell signaling (pale blue) *Nuclei (blue)
OPAL- Colon- *KU80-DNA Damage (red) *E-Cadherin- Epithel (pale blue) *Nuclei (blue)
OPAL- Colon- *KU80-DNA Damage (red) *b Catenin- Epithel (Green) *Nuclei (blue)
OPAL- Colon- *KU80-DNA Damage (red) *E-Cadherin- Epithel (pale blue)
OPAL- Colon- *KU80-DNA Damage (red) *b Catenin- Epithel (Green) *Nuclei (blue)
OPAL- Colon- *KU80-DNA Damage (red) *b Catenin- Epithel (Green) *E-Cadherin (purple) *Nuclei (blue)
Collagen I in diaphragm muscle: -Confocal (left) - 3D (middle) -3D Model (inside tissue section, right)
* aSMA- Marker for stromal cells * STAP- Marker for myofibroblasts * DAPI- Nuclei (blue) Confocal (upper & middle) 3D Model (lower)
* Subcutaneous fat (green, axial) * Visceral fat (red) * CT (Grayscale) 3D Analysis Process (Left) 3D Model (middle, right)
* Brain (blue, coronal) * Tumor (red) * MRI (Grayscale)
* Brain (blue, sagittal) * Tumor (red)
* Lung (green) * Tumor (red) * MRI (grayscale) MRI Scan (left) 3D Model (middle, right)
* Liver- (orange, sagittal) * Tumor (green)
* Liver- (orange) * Tumor (green)
Plant Histology
